Bioassay Technique and Maintenance of Gyropsylla spegazziniana (Lizer, 1719) (Hemiptera: Psylloidea: Aphalaridae) in vitro Conditions
Loeblein, Jaqueline Suelen Alves, Luis Francisco Angeli Coracini, Miryan Denise Araujo Schapovaloff, Maria Elena
Bioassay Technique and Maintenance of Gyropsylla spegazziniana (Lizer, 1719) (Hemiptera: Psylloidea: Aphalaridae) in vitro Conditions
Publicação on-line; 12 ref.; 2 illus.; Summary (En)
ABSTRACT
Gyropsylla spegazziniana is one of the most important pest of yerba mate by sap-sucking that lead
to leaves deformation and there are no insecticides registered for its control. The present study
describes a new technique to be used in bioassays with the insect. Galls were collected from infested
yerba mate plants. In the laboratory, the galls were partially opened and distributed in plastic
containers. The emerged adults were transferred to plastic containers with the perforated bottom
and a central opening in the lid, and subjected to spraying. After that, insects were transferred to
yerba mate seedlings in plastic cages and maintained in laboratory to daily assessment. The use of
a lid with central opening prevented the insects escaping, and avoiding the previous anesthetic to
spraying. The mortality was less than 10% after 10 days. The technique was considered adequate
for maintenance and performance of laboratory bioassays.
Keywords: yerba mate, insect, pest, control, Paraguay tea ampule.
MATE
INSETO
PRAGA DE PLANTA
AMPOLA DA ERVA MATE
Bioassay Technique and Maintenance of Gyropsylla spegazziniana (Lizer, 1719) (Hemiptera: Psylloidea: Aphalaridae) in vitro Conditions
Publicação on-line; 12 ref.; 2 illus.; Summary (En)
ABSTRACT
Gyropsylla spegazziniana is one of the most important pest of yerba mate by sap-sucking that lead
to leaves deformation and there are no insecticides registered for its control. The present study
describes a new technique to be used in bioassays with the insect. Galls were collected from infested
yerba mate plants. In the laboratory, the galls were partially opened and distributed in plastic
containers. The emerged adults were transferred to plastic containers with the perforated bottom
and a central opening in the lid, and subjected to spraying. After that, insects were transferred to
yerba mate seedlings in plastic cages and maintained in laboratory to daily assessment. The use of
a lid with central opening prevented the insects escaping, and avoiding the previous anesthetic to
spraying. The mortality was less than 10% after 10 days. The technique was considered adequate
for maintenance and performance of laboratory bioassays.
Keywords: yerba mate, insect, pest, control, Paraguay tea ampule.
MATE
INSETO
PRAGA DE PLANTA
AMPOLA DA ERVA MATE
BINAGRI