Publicação on-line; 28 ref.; 5 tables; 2 illus.; Summaries (En, Pt)

RESUMO:

Foi estudada a variabilidade isozimática de sete acessos de picão (Bidens pilosa L,
Asteraceae), obtidos nas altitudes de 5,210,475,665,890.1.005 e 1.160 m (acessos A. B, C, D, E. F
e G, respeclivamenle). Os frutos desses acessos foram cultivados na altitude de 665 m. sendo
estudadas as plantas obtidas. Para as folhas e raízes (plantas com 21 dias), tostaram-se sete sistemas
isozimáticos, obtendo três sistemas monomórticos: isocitrato desidrogenaso (IDH), leucina
aminopeptidase (LAP) e chiquimato desidrogenase (SKDH); três polimórbcos: fosfatase ácida (ACP),
esterase (EST) e peroxidase (PO); e um sem resolução satisfatória: malato desidrogenase (MDH),
sendo obtida a melhor resolução para as folhas nos sete sistemas. A análise conjunta dos sistemas
ACP, EST e PO permitiu separar individualmente os acessos A, B, D e G. Observou-se que nos
acessos extremos (A e G) as variações foram mais marcantes O elevado numero de bandas
isozimáticas comuns a todos os acessos (27 das 35 bandas reveladas) pode ler favorecido a adaptação
de B. pilosa a diversas altitudes.


Palavras Chave: Bidens pilosa, picão, isozima, polimorfismo, altitude

ABSTRACT:

Isozyme Characterization of Accesses of Bidens pilosa L. It were studied the
variabilities of isozymes of seven accesses of beggarticks (Bidenspilosa L, Asteraceae) obtained at
5. 210, 475. 665. 890, 1.005 and 1.160 m altitudes (accesses A, B, C. D, E, F and G, respectively)
These accesses fruits were grown at 665 m altitude, and the obtained plants were studied. For leaves
and roots (21 days after emergence) it were tested the isozyme systems From those systems three
were monomorphic: isoertrate dehydrogenase (IDH), leucine aminopeptidase (LAP) and shikimate
dehydrogenase (SKDH); three were polymorphic, acid phosphatase (ACP), esterase (EST) and
peroxidase (PO); and one without a satisfactory resolution, lhat is, the malate dehydrogenase (MDH).
A better resolution occurred for leaves in all systems The analysis of the systems ACP, EST and PO
together, allowed for separating individually the accesses A, B. D and G. It was observed that variation
were more romarkablo in the extreme accesses (A and G). The high number of the isozyme bands
which were common to all accessos (27 from 35 revealed bands) may have favoured B. pilosa
adaptation to those diverse altitudes.


Key Words: Bidenspilosa, beggarticks. isozyme, polymorphism, altitude