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  <controlfield tag="003">BR-BrBNA</controlfield>
  <controlfield tag="005">20240205103736.0</controlfield>
  <controlfield tag="008">240205b2023    bl.ar|pooa||| 00| 0 eng |</controlfield>
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    <subfield code="a">BR-BrBNA</subfield>
    <subfield code="b">eng</subfield>
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  <datafield tag="072" ind1=" " ind2=" ">
    <subfield code="a">F03</subfield>
    <subfield code="b">0810</subfield>
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  <datafield tag="072" ind1=" " ind2=" ">
    <subfield code="a">F60</subfield>
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  <datafield tag="072" ind1=" " ind2=" ">
    <subfield code="a">J11</subfield>
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  <datafield tag="100" ind1=" " ind2=" ">
    <subfield code="a">Cassiano, Cristiano Vasconcelos</subfield>
  </datafield>
  <datafield tag="100" ind1=" " ind2=" ">
    <subfield code="a">Silva, Patricia Pereira da</subfield>
  </datafield>
  <datafield tag="100" ind1=" " ind2=" ">
    <subfield code="a">Pinheiro, Daniel Teixeira</subfield>
  </datafield>
  <datafield tag="100" ind1=" " ind2=" ">
    <subfield code="a">Dias, Denise Cunha Fernandes dos Santos</subfield>
  </datafield>
  <datafield tag="100" ind1=" " ind2=" ">
    <subfield code="a">Morais, Alexandre Augusto de</subfield>
  </datafield>
  <datafield tag="100" ind1=" " ind2=" ">
    <subfield code="a">Nascimento, Warley Marcos</subfield>
  </datafield>
  <datafield tag="245" ind1=" " ind2=" ">
    <subfield code="a">Study of physiological maturity of melon seeds by enzymatic changes</subfield>
  </datafield>
  <datafield tag="500" ind1=" " ind2=" ">
    <subfield code="a">Publica&#xE7;&#xE3;o online; 14 ref.; 6 illus.;  Summaries (En, Pt)</subfield>
  </datafield>
  <datafield tag="520" ind1=" " ind2=" ">
    <subfield code="a">

ABSTRACT - Plant species have different adaptation mechanisms regarding seed survival to desiccation, preventing cellular
destruction during the water loss. The knowledge of these mechanisms is of great importance for the understanding of how
the seed formation/maturation processes and the processes involved in germination occur. Thus, this study aimed to determine
physiological and enzymatic changes during the maturation process of melon seeds of the cultivar BRS Anton, obtained from
fruits at different maturation stages and subjected to post-harvest storage, by the action of total proteins and the enzymes
catalase, peroxidase, and superoxide dismutase. Melon fruits were grown in a greenhouse at Embrapa Vegetables and harvested
at five different times: 30, 45, 60, 75, and 90 days after anthesis (DAA). Thirty fruits were harvested in each season and 15 fruits
had their seeds extracted immediately after harvest and the other 15 fruits were stored for 15 days. After processing and drying,
the seeds were submitted to the following analyses: germination, first count, protein profile analysis, and activity of antioxidant
enzymes (total proteins, peroxidase, catalase, and superoxide dismutase). The activity of the antioxidant enzymes from 60 DAA
under storage showed a more orderly behavior, reaffirming the results of physiological tests. It shows that the seeds at this point
of physiological maturation are well-formed and drying caused no damage to their cell membranes. 


Key words: Protein profile. Antioxidant enzymes. Vigor. Germination.</subfield>
  </datafield>
  <datafield tag="520" ind1=" " ind2=" ">
    <subfield code="a">
RESUMO - As esp&#xE9;cies vegetais apresentam diferentes mecanismos de adapta&#xE7;&#xE3;o no que diz respeito &#xE0; sobreviv&#xEA;ncia das sementes
&#xE0; desseca&#xE7;&#xE3;o, prevenindo a destrui&#xE7;&#xE3;o celular durante a perda de &#xE1;gua. O conhecimento desses mecanismos &#xE9; de grande import&#xE2;ncia
para o entendimento de como ocorre os processos de forma&#xE7;&#xE3;o/matura&#xE7;&#xE3;o de sementes e dos processos envolvidos na germina&#xE7;&#xE3;o.
Dessa forma, o objetivo deste trabalho foi determinar as altera&#xE7;&#xF5;es fisiol&#xF3;gicas e enzim&#xE1;ticas durante o processo de matura&#xE7;&#xE3;o das
sementes de mel&#xE3;o da cultivar &#x201C;BRS Anton&#x201D;, obtidas de frutos em diferentes est&#xE1;dios de matura&#xE7;&#xE3;o e submetidas ao armazenamento
p&#xF3;s-colheita, por meio da a&#xE7;&#xE3;o das prote&#xED;nas totais e das enzimas catalase, peroxidase e super&#xF3;xido dismutase. Os frutos de mel&#xE3;o foram
cultivados em casa de vegeta&#xE7;&#xE3;o na Embrapa Hortali&#xE7;as e colhidos em cinco &#xE9;pocas distintas: aos 30, 45, 60, 75 e 90 dias ap&#xF3;s a antese
(DAA). Em cada &#xE9;poca, foram colhidos 30 frutos, sendo que 15 frutos tiveram suas sementes extra&#xED;das imediatamente ap&#xF3;s a colheita
e 15 frutos foram armazenados por 15 dias. Ap&#xF3;s o beneficiamento e secagem, as sementes foram submetidas &#xE0;s seguintes an&#xE1;lises:
germina&#xE7;&#xE3;o, primeira contagem, an&#xE1;lise do perfil de prote&#xED;nas e atividade de enzimas antioxidantes (prote&#xED;nas totais, peroxidase,
catalase e super&#xF3;xido dismutase). Observou-se que as atividades das enzimas antioxidativas a partir dos 60 DAA sob armazenamento,
apresentou um comportamento mais ordenado reafirmando os resultados dos testes fisiol&#xF3;gicos. Demostrando que as sementes neste
ponto de matura&#xE7;&#xE3;o fisiol&#xF3;gica, encontra-se bem formadas e que a secagem n&#xE3;o provocou danos em suas membranas celulares.


Palavras-chave: Perfil proteico. Enzimas antioxidantes. Vigor. Germina&#xE7;&#xE3;o.</subfield>
  </datafield>
  <datafield tag="650" ind1=" " ind2=" ">
    <subfield code="a">MEL&#xC3;O</subfield>
  </datafield>
  <datafield tag="650" ind1=" " ind2=" ">
    <subfield code="a">SEMENTE</subfield>
  </datafield>
  <datafield tag="650" ind1=" " ind2=" ">
    <subfield code="a">GERMINA&#xC7;&#xC3;O</subfield>
  </datafield>
  <datafield tag="650" ind1=" " ind2=" ">
    <subfield code="a">ARMAZENAMENTO</subfield>
  </datafield>
  <datafield tag="650" ind1=" " ind2=" ">
    <subfield code="a">FISIOLOGIA VEGETAL</subfield>
  </datafield>
  <datafield tag="650" ind1=" " ind2=" ">
    <subfield code="a">ENZIMA</subfield>
  </datafield>
  <datafield tag="773" ind1="0" ind2=" ">
    <subfield code="0">4290</subfield>
    <subfield code="9">275636</subfield>
    <subfield code="d">Fortaleza-CE Universidade Federal do Cear&#xE1;. Centro de Ci&#xEA;ncias Agr&#xE1;rias 2002</subfield>
    <subfield code="o">2023-437436</subfield>
    <subfield code="t">Revista Ci&#xEA;ncia Agron&#xF4;mica (Brazil)</subfield>
    <subfield code="x">0045-6888; 1806-6690 (on-line)</subfield>
    <subfield code="g">v. 54 p. 1-7; (2023)</subfield>
    <subfield code="w">BR2023002353</subfield>
  </datafield>
  <datafield tag="856" ind1=" " ind2=" ">
    <subfield code="u">https://www.scielo.br/j/rca/a/7RG7M5RYzzbVn8C5nZ5SLhj/?format=pdf&amp;lang=en</subfield>
  </datafield>
  <datafield tag="942" ind1=" " ind2=" ">
    <subfield code="c">Anal&#xED;tica</subfield>
  </datafield>
  <datafield tag="999" ind1=" " ind2=" ">
    <subfield code="c">298445</subfield>
    <subfield code="d">298445</subfield>
  </datafield>
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