A separação de flavoróides e naftopiranonas presentes em extratos polares dós capilulos de sempre’vrvas brasileiras loi obtida através da cromatogcafia em contracorrente de gotejamenlo seguida de cromatografias em coluna de PVP e Sephadex LH-20. As substâncias isoladas de extratos etanólicos foram rdentificadas por técnicas espectrométncas e comparadas com dados da literatura Este procedimento levou ao isolamento e idontrficaçào da 9’Gp-D’glcopiranosilpaepalaniina (1J.9- 0-p-D-glicopiranosil(1 —Mjfalopiranosilpaepalanhna (2), além dos flavonóides B-metoxicanferol (3). 3-0-P-D-glicopirano5il-6-meloxicanferol (4), paluletina (5). 3-O-P-D-rutínosilpaluletina (6). 7-O-J3- D-glicopiranosilquercetagetina (7), 5,7É4-1riidfoxi-6,3'-dimeloxiflavona (8) e 5,7.4*4riidraxi-6.3'- dímetoxrllavonoi (9).
Separation of Flavonoids and Naphthopyrones from four Brazilian Paepalanthus Species by Droplet Countercurrent Chromatography. A general procedure was developed for the simultaneous separation of flavonoids and naphthopyrones from the polar extracts ol the capitula Irom Brazilian everlasting plants is described The ethanolic extracts of several species from the Paepa/an/hus genus (Enocaulaceae) were fractionated by droplet countercurrent chromatography followed by column chromatography on pvp and sephadex LH-20 The isolated compounds were identified by spectrometric analysis and comparison with literature data. This approach led to the isolation of 9-O-P-D-giucopyranosylpaepalantine (1), 9-O-p-D-glucopyranosyl (1 —>6)allopyranosylpaepalantine (2). along with the flavonoids 6-methoxykaempferol (3). 3-Op’Dglucopyranosyl-6-methoxykaempferol {4), palulelin (5). 3-Op-D-rijlinosylpatulelrn (6), 7-O-p-Dglucopyranosylquercelagetin (7), 5(7,4'-tnhydroxy’6,3'’dinne1hoKyflavone (8) and 5,7r4'-trihydroxy-6,3'- dimethoxyflavonol (9)