03408nab a2200325 i 4500003000900000005001700009008004100026040001800067072001400085100003200099100003000131100003200161100002500193100003700218100003200255245012000287500006500407520102700472520115801499650001102657650002102668650002102689650001502710650002402725650002602749773019502775856007702970942001603047999001903063BR-BrBNA20240510153412.0240510b2016    bl.mr|pooa||| 00| 0 eng |  aBR-BrBNAbeng  aQ03b6100  aMendonça, Karla Sequeira   aMichael, Geovana Brenner   aNalério, Élen Silveira   aMendonça, Marcelo   aCardoso, Marisa Ribeiro Itapema   aSilva, Wladimir Padilha da   aGenotypic profile of Listeria monocytogenes isolated in refrigerated chickens in southern Rio Grande do Sul, Brazil  aPublicação on-line; 25 ref.; 2 tables; Sumaries (En, Pt)  a
ABSTRACT - Listeria monocytogenes is of notable concern to the food industry, due to its ubiquitous nature and ability to grow in adverse conditions. This study aimed to determine the genotypic profile of L.monocytogenes strains isolated from refrigerated chickens marketed in the southern part of Rio Grande do Sul, Brazil. The strains of L. monocytogenes isolated were characterized by serotyping and Pulsed Field Gel Electrophoresis (PFGE). Three different serotypes (1/2a, 1/2b and 4e) were evaluated by PFGE, and the macrorestriction patterns utilizing enzymes AscI and ApaI, revealed five different pulsotypes. The presence of such varied genotypic profiles demonstrates the prevalence of L. monocytogenes contamination of chicken processing environments, which combined with ineffective cleaning procedures, allowing the survival, adaptation and proliferation of these pathogens, not only in the processing environment, but also in local grocery stores.

Key words: contamination, pathogen, prevalence, serotyping.  a
RESUMO - Listeria monocytogenes é uma notável preocupação para a indústria de alimentos, devido à sua natureza ubíqua e a capacidade de se multiplicar em condições adversas. Este estudo objetivou determinar o perfil genotípico de L. monocytogenes isolada a partir de frangos refrigerados comercializados na região sul do Rio Grande do Sul, Brasil. As cepas de L. monocytogenes foram selecionadas e caracterizadas por sorotipagem e Eletroforese em Gel de Campo Pulsado (PFGE). Três sorotipos diferentes (1/2a, 1/2b e 4e) foram avaliados por PFGE, e a combinação dos padrões de macrorestrição utilizando as enzimas AscI e ApaI revelou cinco diferentes pulsotipos. A presença de diferentes perfis genotípicos demonstra a importância da contaminação no ambiente de processamento de frangos, o qual, juntamente com procedimentos de limpeza ineficazes, permitem a sobrevivência, adaptação e proliferação desses patógenos, não somente no ambiente de processamento, mas também no local de comercialização destes produtos.

Palavras-chave: contaminação, patógeno, prevalência, sorotipagem.  aFRANGO  aREFRIGERAÇÃO  aCONTAMINAÇÃO  aBACTÉRIA  aCOMERCIALIZAÇÃO  aSEGURANÇA ALIMENTAR0 024089314889dSanta Maria-RS Universidade Federal de Santa Maria - Centro de Ciências Rurais 1991o2024-1140tCiência Rural (Brazil)x0103-8478gv. 46(1) p. 132-137; (2016)wBR2024000886  uhttps://www.scielo.br/j/cr/a/N5QCD4qcFvZJfmw6Xr6gCBP/?format=pdf&lang=en  cAnalítica  c299538d299538