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  <titleInfo>
    <title>Determinação do potencial antioxidante, antibacteriano e antifúngico da fração proteica dioscorina de inhame (Dioscorea cayennensis)</title>
  </titleInfo>
  <name type="personal">
    <namePart>Sousa, A.P.</namePart>
    <role>
      <roleTerm authority="marcrelator" type="text">creator</roleTerm>
    </role>
  </name>
  <name type="personal">
    <namePart>Nascimento, E.S.</namePart>
    <role>
      <roleTerm authority="marcrelator" type="text">creator</roleTerm>
    </role>
  </name>
  <name type="personal">
    <namePart>Madruga, M.S.</namePart>
    <role>
      <roleTerm authority="marcrelator" type="text">creator</roleTerm>
    </role>
  </name>
  <name type="personal">
    <namePart>Lima, E.O.</namePart>
    <role>
      <roleTerm authority="marcrelator" type="text">creator</roleTerm>
    </role>
  </name>
  <name type="personal">
    <namePart>Silva, D.F.</namePart>
    <role>
      <roleTerm authority="marcrelator" type="text">creator</roleTerm>
    </role>
  </name>
  <name type="personal">
    <namePart>Gadelha, T.S.</namePart>
    <role>
      <roleTerm authority="marcrelator" type="text">creator</roleTerm>
    </role>
  </name>
  <name type="personal">
    <namePart>Gadelha, C.A.A.</namePart>
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      <roleTerm authority="marcrelator" type="text">creator</roleTerm>
    </role>
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  <genre authority="marc">abstract or summary</genre>
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    <issuance>continuing</issuance>
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  <abstract>

RESUMO: 

O objetivo deste trabalho foi avaliar a ação da fração proteica dioscorina
(FPD) presente nos tubérculos de D. cayennensis frente a cepas de microrganismos 
(bactérias e fungos), bem como determinar o seu potencial antioxidante. O
isolamento da FPD foi realizado com a extração proteica em Tris-HCl 0,05M pH
8,3, seguido de precipitação em Sulfato de Amônio (F45-75). Foi determinada a 
atividade antioxidante pelos métodos de ABTS e DPPH. Devido à presença de maior
teor de aminoácidos específicos (Ácido glutâmico, Ácido Aspártico e Arginina), a
FPD demonstrou um relevante potencial de sequestro de radicais livres, sugerindo
que a interação destes aminoácidos parece atuar em moléculas reativas tornando-
-as estáveis. Por fim, na avaliação da atividade antibacteriana e antifúngica do
extrato total e da FPD frente a diferentes cepas de micro-organismos, foi detectado
potencial antibacteriano entre 50-500 µg/mL de proteína para inibição do crescimento 
da cepa L. monocytogenes.

Palavras-chaves: Proteínas, atividades biológicas, Dioscorina</abstract>
  <abstract>

ABSTRACT: 

Determination of the antioxidant, antibacterial and antifungal potential of the dioscorin 
protein fraction of yam (Dioscorea cayennensis). The
aim of this work was to evaluate the action of the dioscorin protein fraction (DPF)
present in the tubers of D. cayennensis against strains of microorganisms (bacteria
and fungi) as well as to determine their antioxidant potential. Isolation of DPF was
performed with protein extraction in 0.05M Tris-HCl pH 8.3, followed by precipitation
in Ammonium Sulfate (F45-75). The antioxidant activity was estimated by the ABTS
and DPPH methods. Due to the presence of a higher content of specific amino
acids (glutamic acid, aspartic acid and arginine), DPF demonstrated a relevant free
radical sequestration potential, suggesting that the interaction of these amino acids
seems to act on reactive molecules, making them stable. Finally, in the evaluation
of the antibacterial and antifungal activity of the total extract and DPF against different 
strains of microorganisms, an antibacterial potential was detected between
50-500 μg / mL of protein to inhibit the growth of the L. monocytogenes strain.

Keywords: Proteins, Biological activites, Dioscorina.
</abstract>
  <note>Publicação on-line; 47 ref.; 1 table; 1 illus.; Summaries (En, Pt)</note>
  <subject>
    <topic>INHAME</topic>
  </subject>
  <subject>
    <topic>ANTIOXIDANTE</topic>
  </subject>
  <subject>
    <topic>PROTEÍNA VEGETAL</topic>
  </subject>
  <relatedItem type="host">
    <titleInfo>
      <title>Revista Brasileira de Plantas Medicinais (Brazil)</title>
    </titleInfo>
    <originInfo>
      <publisher>Botucatu-SP Instituto de Biociências - Departamento de Química e Bioquímica 1998</publisher>
    </originInfo>
    <identifier>2024-3799</identifier>
    <identifier type="issn">1516-0572</identifier>
    <identifier type="local">BR2024002135</identifier>
    <part>
      <text>v. 19(3) p. 457-465; (2017)</text>
    </part>
  </relatedItem>
  <identifier type="uri">https://www.sbpmed.org.br/admin/files/papers/file_fTQd66hz6jX3.pdf</identifier>
  <location>
    <url>https://www.sbpmed.org.br/admin/files/papers/file_fTQd66hz6jX3.pdf</url>
  </location>
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    <recordContentSource authority="marcorg">BR-BrBNA</recordContentSource>
    <recordCreationDate encoding="marc">240815</recordCreationDate>
    <recordChangeDate encoding="iso8601">20240815135623.0</recordChangeDate>
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      <languageTerm authority="iso639-2b" type="code">por</languageTerm>
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