04027nab a2200265 i 4500003000900000005001700009008004100026040001800067072000800085100001900093100003700112100003700149100004200186245007700228500007600305520151700381520148701898650001603385650002303401650002103424773021903445856007003664942000803734999001903742BR-BrBNA20240913112336.0240913b2020    bl.qr|pooa||| 00| 0 por |  aBR-BrBNAbpor  aF60  aBerla, Sandra   aDamasceno, Ana Paula Lima Guidi   aLeão, Mariella Vieira Pereira   aSantos, Silvana Soléo Ferreira dos   aAção in vitro de extratos de Arrabidaea chica sobre Candida albicans  aPublicação on-line; 20 ref.; 2 tables; 2 illus.; Summaries (En, Pt)  a

RESUMO: 

A comprovação da ação inibitória sobre leveduras do gênero Candida por substâncias
que apresentem baixas ou nenhuma toxicidade ao ser humano, principalmente as extraídas de
plantas já utilizadas na medicina popular, têm sido a intenção de muitas pesquisas, inclusive
essa, cujo objetivo foi determinar a atividade inibitória in vitro de extratos de Arrabidaea
chica sobre C. albicans e quantificar o número de componentes presentes nos extratos. A
concentração inibitória mínima (CIM) de extratos aquoso e hidroalcoólico, autoclavados e
filtrados, foi determinada por diluição em ágar, sobre vinte e cinco cepas de C. albicans e a
separação dos componenetes dos extratos (mistura sólido-líquido) realizada por cromatografia
em camada delgada (CCD). Tanto o extrato hidroalcoólico autoclavado quanto o filtrado inibiram
todas as cepas de C. albicans na concentração de 20%. O extrato aquoso autoclavado inibiu
84% das cepas na concentração de 30% enquanto o filtrado não apresentou efeito inibitório
até a concentração de 50%. A CCD do extrato hidroalcoólico autoclavado evidenciou a
presença de sete componentes e o aquoso autoclavado, dois. Os extratos, hidroalcoólico e
aquoso, apresentaram atividade inibitória sobre C. albicans, entretanto o extrato hidroalcoólico
apresentou desempenho superior e maior número de componentes.

Palavras-chave: Fitoterapia. Candida albicans. Arrabidaea chica. Plantas medicinais.  a

ABSTRACT: 

In vitro effects of Arrabidaea chica on Candida albicans. Evidence of the
inhibitory action on Candida yeasts by substances that present low or none toxicity to humans,
especially substances extracted from plants already used in popular medicine, has been the
intention of many researches. The objective of the present work was to determine the in vitro
inhibitory activity of extracts of Arrabidaea chica on C. albicans and quantify the number of
components present in the extracts. The minimum inhibitory concentration (MIC) of aqueous and
hydroalcoholic extracts, autoclaved and filtered, was determined by agar dilution on twenty-five
strains of C. albicans and the separation of extract components (solid-aqueous mixing), by thin
layer chromatography (TLC). Both the autoclaved hydroalcoholic extract and the filtrated one
inhibited all C. albicans strains, in the concentration of 20%. The autoclaved aqueous extract
inhibited 84% of the strains at the concentration of 30% while the filtrated didn’t showed inhibitory
effect up to a concentration of 50%. The TLC of the autoclaved hydroalcoholic extract evidenced
the presence of seven components and the autoclaved aqueous one, two. The hydroalcoholic
and aqueous extracts of A. chica presented inhibitory activity on C. albicans, however the
hydroalcoholic extract showed superior performance and greater number of components.


Key words: Phytoterapy. Candida albicans. Arrabidaea chica. Medicinal plants.
  aFITOTERAPIA  aCÂNDIDA ALBICANS  aPLANTA MEDICINAL0 035439318027dBotucatu-SP Instituto de Biociências - Departamento de Química e Bioquímica 1998o2024-4761tRevista Brasileira de Plantas Medicinais (Brazil)x1516-0572gv. 22(1) p. 39-44; (2020)wBR2024002583  uhttp://www.sbpmed.org.br/admin/files/papers/file_qpU6QX5Jr3nN.pdf  cANA  c301225d301225