03218nab a2200289 i 4500003000900000005001700009008004100026040001800067072000800085100003200093100003500125100001900160100003100179100003000210100003100240100003100271245009600302500007600398520097000474520111501444650001702559650002102576650002902597650002102626773021002647856007102857BR-BrBNA20240920175815.0240920b2021    bl.qr|pooa||| 00| 0 eng |  aBR-BrBNAbeng  aF60  aLima, Layana Karine Farias   aLustosa, Maria do Carmo Gomes   aRai, Mahendra   aAlmeida, Wanessa Sales de   aAndrade, Nilza Campos de   aFeitosa, Chistiane Mendes   aLima, Sidney Gonçalo de   aChemical composition and anticholinesterase evaluation of aerial parts of Crotalaria retusa  aPublicação on-line; 41 ref.; 4 tables; 8 illus.; Summaries (En, Pt)  a

ABSTRACT: 

Acetylcholinesterase (AChE) inhibition is considered as a promising strategy
for the treatment of neurological disorders. The aim of this research was to determine the
chemical composition of the aerial parts of Crotalaria retusa by gas chromatography coupled
to mass spectrometry (GC-MS), and evaluate their anticholinesterase and cytotoxic potential
against Artemia salina. The main compounds found were trans-hex-2-enal (66.67%) in
fresh leaves; in dry leaves benzene acetaldehyde (23.90%), and in fresh flowers 3-ethyl4-methyl-pent-3-en-2-one (42.80%). The volatile constituents of fresh leaves, hexane
and ethanolic extracts, decoction and dichloromethane fraction showed inhibition against
AChE. The extracts were found to be nontoxic against Artemia salina, which increases the
perspectives of study of the species about new biological activities.

Keywords: Crotalaria retusa; volatile constituents; acetylcholinesterase; chemical composition.  a

RESUMO

Composição química e avaliação anticolinesterase de partes aéreas de Crotalaria
retusa. A inibição da acetilcolinesterase (AChE) é considerada uma estratégia promissora
para o tratamento de distúrbios neurológicos. O objetivo desta pesquisa foi determinar
a composição química das partes aéreas da Crotalaria retusa por cromatografia gasosa
acoplada à espectrometria de massas (CG-EM), e avaliar seu potencial citotóxico e
atividade anticolinesterase. Os principais compostos identificados foram trans-hex-2-
enal (66,67%) nas folhas frescas; fenilacetaldeído (23,90%) nas folhas secas, e nas
flores frescas 3-etil-4-metilpent-3-en-2-ona (42,80%). Os constituintes voláteis das folhas
frescas, hexano e extratos etanólicos, decocção e fração diclorometano apresentaram
inibição da AChE. Os extratos foram encontrados não tóxicos contra Artemia salina, o
que aumenta as perspectivas de estudo da espécie sobre novas atividades biológicas.

Palavras-chave: Crotalaria retusa, Óleo essencial, Composição química, acetilcolinesterase.  aCROTALÁRIA  aÓLEO ESSENCIAL  aCOMPOSIÇÃO QUÍMICA  aPLANTA MEDICINAL0 03543dBotucatu-SP Instituto de Biociências - Departamento de Química e Bioquímica 1998o2024-5009tRevista Brasileira de Plantas Medicinais (Brazil)x1516-0572gv. 23(1) p. 1-11; (2021)wBR2024002630  uhttps://www.sbpmed.org.br/admin/files/papers/file_CMziurGZRaeb.pdf