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  <titleInfo>
    <title>Freezing goat embryos at different developmental stages and quality using ethylene glycol and a slow cooling rate</title>
  </titleInfo>
  <name type="personal">
    <namePart>Fonseca, J.F.</namePart>
    <role>
      <roleTerm authority="marcrelator" type="text">creator</roleTerm>
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  </name>
  <name type="personal">
    <namePart>Batista, R.I.T.P.</namePart>
    <role>
      <roleTerm authority="marcrelator" type="text">creator</roleTerm>
    </role>
  </name>
  <name type="personal">
    <namePart>Souza-Fabjan, J.M.G.</namePart>
    <role>
      <roleTerm authority="marcrelator" type="text">creator</roleTerm>
    </role>
  </name>
  <name type="personal">
    <namePart>Oliveira, M.E.F.</namePart>
    <role>
      <roleTerm authority="marcrelator" type="text">creator</roleTerm>
    </role>
  </name>
  <name type="personal">
    <namePart>Brandão, F.Z.</namePart>
    <role>
      <roleTerm authority="marcrelator" type="text">creator</roleTerm>
    </role>
  </name>
  <name type="personal">
    <namePart>Viana, J.H.M.</namePart>
    <role>
      <roleTerm authority="marcrelator" type="text">creator</roleTerm>
    </role>
  </name>
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  <genre authority="marc">abstract or summary</genre>
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  <abstract>

ABSTRACT - The efficiency of an alternative freezing protocol for goat embryos of different morphology and quality was tested. Fifty-eight embryos on Day 6-7 stage were transferred as fresh or after freeze-thawing (n=29/group). For freezing, embryos were placed into 1.5M ethylene-glycol solution for 10min. During this time, they were loaded in the central part of 0.25mL straw, separated by air bubble from columns containing PBS/BSA 0.4% plus 20% BFS. Straws were then frozen using a freezing machine from 20ºC to –6ºC at a cooling rate of 3ºC/min, stabilization for 15min (seeding after 5min), from –6 C to –32ºC at 0.6 C/min,and plunged into liquid nitrogen. Frozen embryos were thawed for 30s at 37ºC in a water bath. Embryos subjected to fresh transfer were maintained in holding medium (37ºC). Fresh and frozen-thawed embryos were transferred at day 7 post-estrus to 30 recipients. Kidding and kid born rates were similar (P&gt; 0.05), respectively, for recipients receiving fresh (66.7% or 10/15; 55.2% or 16/29) or frozen-thawed (60% or 9/15; 51.7% or 15/29) embryos. The cryopreservation of goat embryos using slow-freezing protocol and 1.5MEG resulted in similar efficiency rates of fresh embryos.


Keywords: embryo survival, embryo transfer, goat, slow freezing</abstract>
  <abstract>

RESUMO - Este estudo testou a eficiência de protocolo alternativo de criopreservação de embriões caprinos de diferentes qualidades morfológicas. Foram utilizados 58 embriões, coletados entre o sexto e o sétimo dia do ciclo estral (n=29/grupo). Embriões congelados passaram por solução 1,5M etilenoglicol por 10min e foram aspirados durante esse tempo para parte central de palheta 0,25mL, separada por bolhas de ar de colunas contendo PBS 0,4% BSA e 20% SFB. As palhetas foram congeladas em máquina de congelação de 20ºC a -6ºC, com taxa de resfriamento de 3ºC/min, estabilização por 15min (seeding após 5min), -6ºC a -32ºC a 0,6ºC/min, e imersas em nitrogênio líquido. Os embriões foram descongelados por 30s a 37ºC, em água. Embriões frescos foram mantidos em solução de manutenção (37ºC). Embriões frescos e congelados/descongelados foram transferidos para 30 receptoras no sétimo dia do ciclo estral. A taxa de partos e a de crias nascidas (respectivamente) foram similares (P&gt;0,05) para receptoras recebendo embriões frescos (66,7% ou 10/15; 55,2% ou 16/29) ou congelados/descongelados (60,0% ou 9/15; 51,7% ou 15/29). O protocolo de criopreservação de embriões utilizado no presente estudo resultou em índices de eficiência semelhantes aos de embriões frescos.


Palavras-chave: caprinos, sobrevivência embrionária, taxa de resfriamento lenta, transferência de embriões</abstract>
  <note>Publicação online; 28 ref.; 2 tables; 2 illus.; Summaries (En, Pt)</note>
  <subject>
    <topic>CABRA</topic>
  </subject>
  <subject>
    <topic>REPRODUÇÃO ANIMAL</topic>
  </subject>
  <subject>
    <topic>CRIOPRESERVAÇÃO</topic>
  </subject>
  <subject>
    <topic>TRANSFERÊNCIA DE EMBRIÃO</topic>
  </subject>
  <subject>
    <topic>TECNOLOGIA APROPRIADA</topic>
  </subject>
  <relatedItem type="host">
    <titleInfo>
      <title>Arquivo Brasileiro de Medicina Veterinária e Zootecnia (Brazil)</title>
    </titleInfo>
    <originInfo>
      <publisher>Belo Horizonte-MG Universidade Federal de Minas Gerais - Escola de Veterinaria 1983</publisher>
    </originInfo>
    <identifier>2024-5011</identifier>
    <identifier type="issn">0102-0935</identifier>
    <identifier type="local">BR2024002723</identifier>
    <part>
      <text>v. 70(5) p. 1489-1496. (2018)</text>
    </part>
  </relatedItem>
  <identifier type="uri">https://www.scielo.br/j/abmvz/a/MDtK8843m4VpBdD8CMcjJ5z/?format=pdf&amp;lang=en</identifier>
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    <url>https://www.scielo.br/j/abmvz/a/MDtK8843m4VpBdD8CMcjJ5z/?format=pdf&amp;lang=en</url>
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    <recordCreationDate encoding="marc">240926</recordCreationDate>
    <recordChangeDate encoding="iso8601">20240926123540.0</recordChangeDate>
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      <languageTerm authority="iso639-2b" type="code">eng</languageTerm>
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