03974nab a2200325 i 4500003000900000005001700009008004100026040001800067072001400085072000800099100001800107100002300125100002600148100002200174100002100196100001900217245011800236500007500354520129100429520146601720650001003186650002603196650002403222650003303246650002603279773023603305856008003541942000803621999001903629BR-BrBNA20240926123540.0240926b2018    bl.|r|pooa||| 00| 0 eng |  aBR-BrBNAbeng  aL53b5250  aL70  aFonseca, J.F.  aBatista, R.I.T.P.   aSouza-Fabjan, J.M.G.   aOliveira, M.E.F.   aBrandão, F.Z.   aViana, J.H.M.   aFreezing goat embryos at different developmental stages and quality using ethylene glycol and a slow cooling rate  aPublicação online; 28 ref.; 2 tables; 2 illus.; Summaries (En, Pt)  a

ABSTRACT - The efficiency of an alternative freezing protocol for goat embryos of different morphology and quality was tested. Fifty-eight embryos on Day 6-7 stage were transferred as fresh or after freeze-thawing (n=29/group). For freezing, embryos were placed into 1.5M ethylene-glycol solution for 10min. During this time, they were loaded in the central part of 0.25mL straw, separated by air bubble from columns containing PBS/BSA 0.4% plus 20% BFS. Straws were then frozen using a freezing machine from 20ºC to –6ºC at a cooling rate of 3ºC/min, stabilization for 15min (seeding after 5min), from –6 C to –32ºC at 0.6 C/min,and plunged into liquid nitrogen. Frozen embryos were thawed for 30s at 37ºC in a water bath. Embryos subjected to fresh transfer were maintained in holding medium (37ºC). Fresh and frozen-thawed embryos were transferred at day 7 post-estrus to 30 recipients. Kidding and kid born rates were similar (P> 0.05), respectively, for recipients receiving fresh (66.7% or 10/15; 55.2% or 16/29) or frozen-thawed (60% or 9/15; 51.7% or 15/29) embryos. The cryopreservation of goat embryos using slow-freezing protocol and 1.5MEG resulted in similar efficiency rates of fresh embryos.


Keywords: embryo survival, embryo transfer, goat, slow freezing  a

RESUMO - Este estudo testou a eficiência de protocolo alternativo de criopreservação de embriões caprinos de diferentes qualidades morfológicas. Foram utilizados 58 embriões, coletados entre o sexto e o sétimo dia do ciclo estral (n=29/grupo). Embriões congelados passaram por solução 1,5M etilenoglicol por 10min e foram aspirados durante esse tempo para parte central de palheta 0,25mL, separada por bolhas de ar de colunas contendo PBS 0,4% BSA e 20% SFB. As palhetas foram congeladas em máquina de congelação de 20ºC a -6ºC, com taxa de resfriamento de 3ºC/min, estabilização por 15min (seeding após 5min), -6ºC a -32ºC a 0,6ºC/min, e imersas em nitrogênio líquido. Os embriões foram descongelados por 30s a 37ºC, em água. Embriões frescos foram mantidos em solução de manutenção (37ºC). Embriões frescos e congelados/descongelados foram transferidos para 30 receptoras no sétimo dia do ciclo estral. A taxa de partos e a de crias nascidas (respectivamente) foram similares (P>0,05) para receptoras recebendo embriões frescos (66,7% ou 10/15; 55,2% ou 16/29) ou congelados/descongelados (60,0% ou 9/15; 51,7% ou 15/29). O protocolo de criopreservação de embriões utilizado no presente estudo resultou em índices de eficiência semelhantes aos de embriões frescos.


Palavras-chave: caprinos, sobrevivência embrionária, taxa de resfriamento lenta, transferência de embriões  aCABRA  aREPRODUÇÃO ANIMAL  aCRIOPRESERVAÇÃO  aTRANSFERÊNCIA DE EMBRIÃO  aTECNOLOGIA APROPRIADA0 016439344677dBelo Horizonte-MG Universidade Federal de Minas Gerais - Escola de Veterinaria 1983o2024-5011tArquivo Brasileiro de Medicina Veterinária e Zootecnia (Brazil)x0102-0935gv. 70(5) p. 1489-1496. (2018)wBR2024002723  uhttps://www.scielo.br/j/abmvz/a/MDtK8843m4VpBdD8CMcjJ5z/?format=pdf&lang=en  cANA  c327842d327842