03633nab a2200265 i 4500003000900000005001700009008004100026040001800067072001400085100002200099100002900121100002000150100001900170100002800189100003600217245013000253500007600383520243600459650001402895650002802909650002402937650000902961773031802970856007903288BR-BrBNA20250210160931.0250210b2021    bl.qr|pooa||| 00| 0 eng |  aBR-BrBNAbeng  aH20b2140  aMezzomo, Ricardo   aRolim, Jéssica Mengue   aPoletto, Tales   aWalker, Clair   aMilanesi, Paola Mendes   aMuniz, Marlove Fátima Brião  aMolecular characterization and vegetative compatibility groups of Fusarium spp. pathogenic to Ilex paraguariensis A. St.-Hil.  aPublicação on-line; 30 ref.; 3 tables; 4 illus.; Summaries (En, Pt)  a

ABSTRACT

In the central-western and southern regions of Brazil, the cultivation of yerba mate (Ilex paraguariensis) is
considered an important source of forest extraction, besides providing an alternative income, especially
for families. The yerba mate, like other forest species, is infected by several pathogens, such as fungi
of the genus Fusarium, causing root rot. Pathogens have wide genetic variability, even within a single
genus and species, resulting from adaptations to their environments. This study aimed at characterizing
molecularly isolated Fusarium spp. through the sequencing of the ITS and β-tubulin genome regions,
identifying the species level as well as the formation of vegetative compatibility groups (VCG) among
these isolates of the root rot pathogen in yerba mate. The sequencing of the ITS and β-tubulin regions,
defined the separation between the seven pathogenic isolates of F. solani and F. oxysporum. For the same
isolates, the technique to obtain nit mutants was suitable for the phenotypic classification, obtaining
five VCGs for species of Fusarium, root rot in yerba mate.

Keywords: ITS; β-tubulin; nit


RESUMO

Nas regiões Centro-Oeste e Sul do Brasil, a cultura da erva-mate (Ilex paraguariensis) constitui-se uma
importante fonte de extrativismo florestal, além de diversificar a renda, principalmente na agricultura
familiar. A erva-mate, como outras espécies florestais, é infectada por diversos patógenos, como o
gênero Fusarium, causador da podridão-de-raízes. Os patógenos possuem ampla variabilidade genética,
mesmo dentro de um único gênero e dentro de uma única espécie, resultado de adaptações ao meio
em que vivem. Este estudo teve como objetivo avaliar o sequenciamento das regiões do genoma ITS e
β-tubulina na identificação em nível de espécie dos isolados de Fusarium spp. patogênicos à erva-mate,
e identificar a formação de grupos de compatibilidade vegetativa (VCG) entre os isolados de Fusarium
spp. patogênicos à erva-mate. O sequenciamento das regiões ITS e β-tubulina consolidou a separação
entre os sete isolados patogênicos em F. solani e F. oxysporum. Para esses isolados, a técnica para
obtenção de mutantes nit foi adequada para a classificação fenotípica, fundamentais nos testes de VCG
para espécies de Fusarium, sendo obtidos cinco VCG.

Palavras-chave: ITS; β-tubulina; nit  aFUSARIUM   aVARIAÇÃO GENÉTICA  aILEX PARAGUARIENSIS  aMATE0 04065dSanta Maria-RS Universidade Federal de Santa Maria - Centro de Pesquisas Florestais.Departamento de Ciências Florestais. Programa de Pós-Graduação em Engenharia Florestal 1991o2024-8033tCiência Florestal (Brazil)x0103-9954; 1980-5098 (on-line)gv. 31(2) p. 846-862; (Apr-Jun 2021)wBR2023001865  uhttps://www.scielo.br/j/cflo/a/vkHNqTPZgcnYPjjvvnPKG3H/?format=pdf&lang=en