02044nab a2200253 i 4500003000900000005001700009008004100026040001800067072001400085072000900099100003200108100003400140100003500174100003100209245013800240500006000378520102600438650000901464650001101473650002001484650002401504773018101528856008101709BR-BrBNA20260409051635.0260409b2019    bl.qr|pooa||| 00| 0 eng |  aBR-BrBNAbeng  aH10b7900  b2140  aLoeblein, Jaqueline Suelen   aAlves, Luis Francisco Angeli   aCoracini, Miryan Denise Araujo  aSchapovaloff, Maria Elena   aBioassay Technique and Maintenance of Gyropsylla spegazziniana (Lizer, 1719) (Hemiptera: Psylloidea: Aphalaridae) in vitro Conditions  aPublicação on-line; 12 ref.; 2 illus.; Summary (En)  a

ABSTRACT

Gyropsylla spegazziniana is one of the most important pest of yerba mate by sap-sucking that lead
to leaves deformation and there are no insecticides registered for its control. The present study
describes a new technique to be used in bioassays with the insect. Galls were collected from infested
yerba mate plants. In the laboratory, the galls were partially opened and distributed in plastic
containers. The emerged adults were transferred to plastic containers with the perforated bottom
and a central opening in the lid, and subjected to spraying. After that, insects were transferred to
yerba mate seedlings in plastic cages and maintained in laboratory to daily assessment. The use of
a lid with central opening prevented the insects escaping, and avoiding the previous anesthetic to
spraying. The mortality was less than 10% after 10 days. The technique was considered adequate
for maintenance and performance of laboratory bioassays.

Keywords: yerba mate, insect, pest, control, Paraguay tea ampule.  aMATE  aINSETO  aPRAGA DE PLANTA  aAMPOLA DA ERVA MATE0 02929dRio de Janeiro-RJ Instituto de Florestas - UFRRJ 1994o2025-0451tFloresta e Ambiente (Brazil)x1415-0980  /  ISSN 2179-8087 0nlinegv. 26(3) p. 1-4; (2019)wBR2026000320  uhttps://www.scielo.br/j/floram/a/ktt9fQqq57DnCF5s6MFwsjS/?format=pdf&lang=en