Loeblein, Jaqueline Suelen creator Alves, Luis Francisco Angeli creator Coracini, Miryan Denise Araujo creator Schapovaloff, Maria Elena creator text periodical abstract or summary bl. continuing eng ABSTRACT Gyropsylla spegazziniana is one of the most important pest of yerba mate by sap-sucking that lead to leaves deformation and there are no insecticides registered for its control. The present study describes a new technique to be used in bioassays with the insect. Galls were collected from infested yerba mate plants. In the laboratory, the galls were partially opened and distributed in plastic containers. The emerged adults were transferred to plastic containers with the perforated bottom and a central opening in the lid, and subjected to spraying. After that, insects were transferred to yerba mate seedlings in plastic cages and maintained in laboratory to daily assessment. The use of a lid with central opening prevented the insects escaping, and avoiding the previous anesthetic to spraying. The mortality was less than 10% after 10 days. The technique was considered adequate for maintenance and performance of laboratory bioassays. Keywords: yerba mate, insect, pest, control, Paraguay tea ampule. Publicação on-line; 12 ref.; 2 illus.; Summary (En) MATE INSETO PRAGA DE PLANTA AMPOLA DA ERVA MATE Rio de Janeiro-RJ Instituto de Florestas - UFRRJ 1994 2025-0451 1415-0980 / ISSN 2179-8087 0nline BR2026000320 v. 26(3) p. 1-4; (2019) https://www.scielo.br/j/floram/a/ktt9fQqq57DnCF5s6MFwsjS/?format=pdf&lang=en https://www.scielo.br/j/floram/a/ktt9fQqq57DnCF5s6MFwsjS/?format=pdf&lang=en BR-BrBNA 260409 20260409051635.0 eng