Silva, Karol Buuron da creator Reiniger, Lia Rejane Silveira creator Stefanel, Charlene Moro creator Rabaiolli, Silvia Machado dos Santos creator text periodical abstract or summary bl. continuing eng Abstract The objective of this study was to evaluate the effect of sucrose and substrates on the acclimatization of micropropagated Luehea divaricata plants. First, the effect of sucrose on in vitro growth and development of nodal segments in Woody Plant Medium reduced to its half concentration was tested. After that, these shoots were then acclimatized and the combined effect of sucrose, as previously described, plus the commercial substrates vermiculite, MecPlant® or H-decker® were tested. In the in vitro culture, root and leaf formation were superior (44%, 8.44 leaves) with 20 g L-1 sucrose, and then the presence of sucrose continued to be more efficient in the acclimatization with the MecPlant® or H-decker® commercial substrates. Sucrose at 20 g L-1 is more efficient for in vitro leaf and root formation, and the MecPlant® or H-decker® substrates may be used in acclimatization. Keywords: micropropagation, forest species, carbohydrate. Publicação on-line; 27 ref.; 1 table; 4 illus.; Summary (En) LUEHEA DIVARICATA AÇOITA CAVALO MICROPROPAGAÇÃO ESSÊNCIA FLORESTAL CARBOIDRATO SACAROSE Rio de Janeiro-RJ Instituto de Florestas - UFRRJ 1994 2025-0455 1415-0980 / ISSN 2179-8087 0nline BR2026001222 v. 27(1) p. 1-9; (2020) https://www.scielo.br/j/floram/a/z9s5jKqPg6tYSHtgG3zfWpJ/?format=pdf&lang=en https://www.scielo.br/j/floram/a/z9s5jKqPg6tYSHtgG3zfWpJ/?format=pdf&lang=en BR-BrBNA 260512 20260512141009.0 eng