02260nab a2200301 i 4500003000900000005001700009008004100026040001800067072001400085072000900099100002000108100003700128100003400165100002300199100002900222245010700251500007000358520113200428650001401560650002501574650001501599650002401614650002301638773018901661856008101850942000801931999001901939BR-BrBNA20260520175006.0260520b2020    bl.qr|pooa||| 00| 0 eng |  aBR-BrBNAbeng  aH20b4120  b1240  aPoletto, Tales   aMuniz, Marlove Fátima Brião   aFantinel, Vinícius Spolaor   aHarakava, Ricardo   aRolim, Jéssica Mengue   aCharacterization and Pathogenicity of Fusarium oxysporum Associated with Carya illinoinensis Seedlings  aPublicação on-line; 38 ref.; 2 tables; 2 illus.; Summary (En)  a

Abstract

Pecan cultivation in Brazil has gained increasingly economic importance in the southern part of the country.
The objective of this work was to identify the pathogen that causes root rot in Carya illinoinensis seedlings in the nursery,
through morphological, molecular and pathogenicity tests. Fungi from the genus Fusarium found in symptomatic
roots of pecan seedlings were isolated in potato dextrose agar (PDA) culture medium and purified. Morphological
characterization was performed in PDA and carnation leaf agar (CLA) media. For the molecular characterization,
the genomic segments ITS (internal transcribed spaces), β-tub (beta-tubulin), and tef1-α (elongation fact 1-alpha)
were sequenced. The pathogenicity test was performed on healthy seedlings in the greenhouse. The combination of
morphological and molecular characters was fundamental for identification of the species, showing that the most
suitable genes for identification were ITS and tef1-α. The causative agent of root rot in C. illinoinensis seedlings in
the nursery was identified as Fusarium oxysporum.

Keywords: etiology, root rot, pecan  aETIOLOGIA  aPODRIDÃO RADICULAR  aNOZ PECÃ  aCARYA ILLINOINENSIS  aFUSARIUM OXYSPORUM0 029299347957dRio de Janeiro-RJ Instituto de Florestas - UFRRJ 1994o2025-0456tFloresta e Ambiente (Brazil)x1415-0980  /  ISSN 2179-8087 0nlinegv. 27(2) p. 1-9; (2020)wBR2026001248  uhttps://www.scielo.br/j/floram/a/7NV4SfJBJVsdsgrvjbDLq3B/?format=pdf&lang=en  cANA  c341878d341878