000			03144nab a2200337 i 4500
003			BR-BrBNA
005			20250123125825.0
008			250123b2016 bl.|r|pooa||| 00| 0 por |
040			_aBR-BrBNA _bpor
072			_aL73 _b5400
072			_aL70
100			_aVasconcelos, T.C.B.
100			_aMachado, L.C.
100			_aAbrantes, T.R.
100			_aMenezes, R.C.
100			_aMadeira, M.F.
100			_a Ferreira, L.C.
100			_aFigueiredo, F.B.
245			_aAvaliação da confiabilidade entre dois observadores em exames citopatológico e imunocitoquímico de aspirado de medula óssea no diagnóstico da leishmaniose visceral canina
500			_aPublicação online; 13 ref.; Summary (En)
520			_a ABSTRACT - Visceral leishmaniasis is a zoonosis in which the dog appears as the main source of infection in urban areas. Its diagnosis is complex and the cytopathological exam is a fast and cheap alternative to parasite direct visualization and its sensitivity can be increased by immunocytochemistry, though with a higher cost. The accuracy of such methods is dependent on the microscopist's experience and therefore, this study evaluated the reliability of such techniques between two observers, from bone marrow aspirates of 50 dogs from an endemic area for the disease. The parasitological culture in Novy-MacNeal-Nicolle medium was used as the reference standard. Among the main findings, the sensitivities obtained by observers I and II were respectively 62.5% and 37.5%, while specificities were 81.1% and 100%. On immunocytochemistry evaluation, the sensitivity was 0% for both evaluators and the specificity 97.3% and 100%. The agreement between evaluators was weak (κ = 0.167) for the cytopathological test and it could not be evaluated for immunocytochemistry, for which there was no detection by the evaluator II. The agreements among the diagnostic methods and the standard reference for the observer I were reasonable (κ = 0.364) for cytopathological examination and bad (κ = -0.041) for immunocytochemistry. For observer II, such agreement could be assessed only for the cytopathological test, being moderate (κ = 0.497). The results point to the possible expertise difference between evaluators, with the evaluator II demonstrating greater experience when interpreting the citopathological test. Although there was the expected sensitivity increase with immunocytochemistry, the technique used in this study was not effective for the diagnosis of infection, regardless of the evaluator. Keywords: dog, Leishmania infantum, diagnostic, reliability
650			_aCÃO
650			_aDOENÇA ANIMAL
650			_aLEISHMANIOSE
650			_aDIAGNÓSTICO
650			_aVETERINÁRIA
773	0		_01643 _9347391 _dBelo Horizonte-MG Universidade Federal de Minas Gerais - Escola de Veterinaria 1983 _o2024-8404 _tArquivo Brasileiro de Medicina Veterinária e Zootecnia (Brazil) _x0102-0935 _gv. 68(3) p. 821-824; (2016) _wBR2024004515
856			_uhttps://www.scielo.br/j/abmvz/a/5GqjrydFgBBXbTYhQfQWSfP/?format=pdf&lang=pt
942			_cANA
999			_c329655 _d329655