000			02550nab a2200337 i 4500
003			BR-BrBNA
005			20250506163818.0
008			250506b2020 bl.mr|pooa||| 00| 0 eng |
040			_aBR-BrBNA _beng
072			_aH20 _b1420
100			_aMedeiros, Layla de Sá Andrade
100			_aOliveira, Izaías Araújo de
100			_aKitajima, Elliot Watanabe
100			_aEiras, Marcelo
100			_aPereira, Helen Jesus
100			_aRibeiro, Simone Graça
100			_aMatos, Kedma da Silva
100			_aBeserra Júnior, José Evando Aguiar
245			_aA survey of RNA genome viruses in Lima bean crops of Northeastern Brazil
500			_aPublicação on-line; 23 ref.; 4 illus; Sumaries (En)
520			_a ABSTRACT: Lima bean plants showing mosaic and leaf distortion symptoms, similar to those induced by viruses, were observed in several production areas in the states of Ceará and Piauí, Northeastern Brazil. The aim of this study was to identify RNA genome viruses that infect lima bean. Field research was conducted from 2017 to 2018. Fifty-five symptomatic samples were randomly collected from seven properties in five municipalities and tested by RT-PCR and DNA sequencing with specific or universal primers for two viruses and two virus groups. Four virus species were identified: cucumber mosaic virus (CMV), cowpea mild mottle virus (CPMMV), cowpea aphid-borne mosaic virus (CABMV) and cowpea severe mosaic virus (CPSMV), which had infection levels of 21.8, 52.7, 47.2, and 1.8%, respectively, as well as double and triple infections. The CMV isolates belonged to subgroup IA. The CPMMV isolates had high nucleotide identity with CPMMV isolates from Brazil, USA and Mexico. The CABMV isolates showed moderate nucleotide identity with Brazilian isolates. Only one sample was infected with CPSMV. This is the first record of CPMMV and CPSMV naturally infecting lima bean. Approaches to virus control are discussed. Key words: carlavirus, comovirus, cucumovirus, Phaseolus lunatus, potyvirus, virus identification.
650			_aFEIJÃO FAVA
650			_aDOENÇA DE PLANTA
650			_aVÍRUS
650			_aMOSAICO DOURADO
650			_aDEFESA VEGETAL
773	0		_01365 _9344637 _dCampinas-SP Instituto Agronômico - Coordenadoria da Pesquisa Agropecuária 1941 _o2024-4959 _tBragantia (Brazil) _x0006-8705 _gv. 49 p. 407-416 ; (2020) _wBR2025001018
856			_uhttps://www.scielo.br/j/brag/a/fpLBBcX8jPCfFM3jkgxDTRK/?format=pdf&lang=en
942			_cANA
999			_c331360 _d331360