000			02152nab a2200289 i 4500
003			BR-BrBNA
005			20260409051635.0
008			260409b2019 bl.qr|pooa||| 00| 0 eng |
040			_aBR-BrBNA _beng
072			_aH10 _b7900
072			_b2140
100			_aLoeblein, Jaqueline Suelen
100			_aAlves, Luis Francisco Angeli
100			_aCoracini, Miryan Denise Araujo
100			_aSchapovaloff, Maria Elena
245			_aBioassay Technique and Maintenance of Gyropsylla spegazziniana (Lizer, 1719) (Hemiptera: Psylloidea: Aphalaridae) in vitro Conditions
500			_aPublicação on-line; 12 ref.; 2 illus.; Summary (En)
520			_a ABSTRACT Gyropsylla spegazziniana is one of the most important pest of yerba mate by sap-sucking that lead to leaves deformation and there are no insecticides registered for its control. The present study describes a new technique to be used in bioassays with the insect. Galls were collected from infested yerba mate plants. In the laboratory, the galls were partially opened and distributed in plastic containers. The emerged adults were transferred to plastic containers with the perforated bottom and a central opening in the lid, and subjected to spraying. After that, insects were transferred to yerba mate seedlings in plastic cages and maintained in laboratory to daily assessment. The use of a lid with central opening prevented the insects escaping, and avoiding the previous anesthetic to spraying. The mortality was less than 10% after 10 days. The technique was considered adequate for maintenance and performance of laboratory bioassays. Keywords: yerba mate, insect, pest, control, Paraguay tea ampule.
650			_aMATE
650			_aINSETO
650			_aPRAGA DE PLANTA
650			_aAMPOLA DA ERVA MATE
773	0		_02929 _9347952 _dRio de Janeiro-RJ Instituto de Florestas - UFRRJ 1994 _o2025-0451 _tFloresta e Ambiente (Brazil) _x1415-0980 / ISSN 2179-8087 0nline _gv. 26(3) p. 1-4; (2019) _wBR2026000320
856			_uhttps://www.scielo.br/j/floram/a/ktt9fQqq57DnCF5s6MFwsjS/?format=pdf&lang=en
942			_cANA
999			_c341297 _d341297