000			02943nab a2200313 i 4500
003			BR-BrBNA
005			20230922123210.0
008			230807b2020 bl.ar|pooa||| 00| 0 eng |
040			_aBR-BrBNA _beng
072			_aH60
072			_aH20
100			_aAkbar, M.
100			_aSherazi, I.N.
100			_aKhalil, T.
100			_aIqbal, M.S.
100			_aAkhtar, S.
100			_aKhan, S.N.
245			_aIdentification of antifungal compounds from slender amaranth
500			_aPublicação on-line; 39 ref.; 1 table; 2 illus.; Summary (En)
520			_a ABSTRACT Background: Antifungal activity of slender amaranth (Amaranthus viridis L.) is well documented but such studies are scarce in Pakistan, especially against plant pathogens. It was hypothesized that A. viridis has antifungal activity against fungal phyto-pathogens also. Objective: Identification of antifungal constituents from leaf extracts of A. viridis. Methods: Different organic solvent extracts of A. viridis leaves were evaluated against 5 plant pathogenic fungal species viz. Alternaria alternata, Aspergillus flavus, Drechslera australiensis, Fusarium oxysporum and Macrophomina phaseolina. Antifungal activity of A. viridis was determined by serial dilution method. Six levels (0, 5, 10, 15, 20 and 25 mg mL-1) of treatments of each n-hexane, chloroform and ethyl acetate were employed against all fungal species in a Completely Randomised Design (CRD). Results: Generally, all organic solvent extracts reduced the fungal biomass significantly with the increase in extract concentration but ethyl acetate leaf fraction exhibited pronounced activity and reduced the fungal growth up to 44% in A. alternata, 39% in A. flavus, 48% in D. australiensis, 48% in F. oxysporum and 45% in M. phaseolina. Gas Chromatography Mass Spectrometry (GCMS) analysis of ethyl acetate leaf fraction revealed 09 compounds. Out of these 9 compounds, one compound identified as 1,2- Benzenedicarboxylic acid, mono (2-ethylhexyl) ester) showed 58.5% peak value. Conclusions: It was concluded that 1,2- Benzenedicarboxylic acid, mono (2-ethylhexyl) ester) being in the highest concentration in the ethyl acetate leaf fraction of A. viridis may be responsible for antifungal activity. This compound can serve as structural analog to develop ecofriendly fungicides. Keywords: Amaranthus viridis antifungal GC-MS plant pathogenic slender amaranth
650			_aAMARANTHUS VIRIDES
650			_aDOENÇA DE PLANTA
650			_aFUNGICIDA
650			_aCROMATOGRAFIA GASOSA
773	0		_0886 _91817 _dLondrina-PR : Sociedade Brasileira da Ciência das Plantas Daninhas, 1978 _o2023-031024 _tPlanta Daninha (Brazil) _x0100-8358; 1806-9681 (on-line) _gv. 38 p. 1-8; (2020) _wBR2023000836
856			_uhttps://www.scielo.br/j/pd/a/LrBtFmhVCY6Tgx3gCfy4VYD/?format=pdf&lang=en
942			_cAnalítica
999			_c9748 _d9748